Paper chromatography coursework

Paper Chromatography Experiment: Middle School Science Lesson

Make a horizontal line with the marker approximately 1 inch from the bottom of the paper strip. Attach the top of the paper to a pencil with tape. Lay the pencil across the top of the cup so that the paper strip hangs down into the water, but make sure that the bottom edge of the strip barely touches the water. The ink line should not be in the water.

Chromatography Experiment

Allow the water to travel up the paper for several minutes. The ink will become blurred, and the different dyes in the ink will separate. The result will be a pattern of different pigments, and the pattern will be different for each brand of marker, because they use different combinations and colors in their ink.

Let the paper strips dry and staple them to the data sheet.

Paper Chromatography Experiment Report

If there is time, students can try it with other colored markers besides black. Have students answer the following questions on their data sheet:.


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What colors make up the black ink? Were the colors different for the different brands of marker you tested? Describe the colors you saw for each brand of marker. What is the solvent? What is the solute? Bright Hub Education.

Paper Chromatography Essay Example | Graduateway

Skip to content. How Paper Chromatography Works Paper chromatography is a method that is used to separate out materials from a mixture. Paper Chromatography Lab Procedure Materials: Several different brands of black water-soluble markers Sharpies will not work, they are not water-soluble Coffee filters cut into strips approximately 1 inch wide and inches long Paper or plastic cups Pencils Tape Water Method: Choose three different brands of black marker Write each brand name at the top of a paper strip in pencil.

Make a horizontal line with the marker approximately 1 inch from the bottom of the paper strip Attach the top of the paper to a pencil with tape.

How Paper Chromatography Works

Take the paper out and analyze the color separation once the water runs up to within an inch from the top of the paper. A leaf has several pigments that can be separated using chromatography.


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Grind finely cut leaves, along with a small pinch of sand and a solvent such as propanone, using a mortar and pestle for three minutes. Draw a pencil line three centimeters from the bottom of a strip of chromatography paper and use a fine glass tube to place seven spots of this leaf mixture on the line.

Wait for each spot to dry before adding another one directly on top of the previous one.


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  4. Place the bottom edge of the paper in a beaker with propanone just covering the bottom. Remove the paper when the propanone is near the top or has stopped moving up the paper.

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    Because propanone is highly flammable, wear protective goggles when doing this experiment. Draw a pencil line across the bottom of a chromatography paper strip, two centimeters from the bottom.

    Place spots of food coloring on the line, making sure the colors are concentrated in a small area and at least three centimeters apart. Dissolve milligrams of table salt into milliliters of water and pour just enough of this mixture to cover the base of the beaker or jar. Wrap the paper into a cylinder shape and secure it with a staple before placing it in the beaker.

    go to link Remove the paper from the beaker when the solution has moved to within two centimeters from the top. Gina Battaglia has written professionally since She served as an assistant editor for the "International Journal of Sports Medicine" and coauthored a paper published in the "Journal of Strength and Conditioning Research.

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